摘要目的 探讨高迁移率族蚩白B1(HMGB1)在白细胞介素-2(IL-2)转录表达信号调控机制中的可能作用.方法首先将HMGB1和NFAT2质粒共同转染Hela细胞,同时转染IL-2报告基因,逐步增加HMGB1的转染剂量,检测IL-2报告基因的表达活性.观察HMGB1质粒用量埘IL-2报告基凶活性的影响;然后应用sRNAi质粒对内源性及外源性HMGB1表达进行特异性抑制,观察对IL-2报告基因活性的影响,以期从反而论证HMGB1可促进IL-2转录表达.结果 在Hela细胞中,随着HMGB1质粒转染剂量增加,IL-2报告基因活性增加了2.12倍(P<0.01).应用sRNAi抑制293T细胞中外源性以及Hela细胞中内源性HMGB1表达水平后,IL-2报告基因活性分别下降1.7倍和4.76倍(P<0.05或P<0.01).结论 HMGB1在NFAT2介导IL-2报告基冈转录表达的信号调控过程中发挥重要作用.

abstractsObejective To investigate the potential role of high mobility group box-1 protein (HMGB1) in transcription and expression of interleukin (IL)-2 mediated by nuclear factor of activating T cells 2 (NFAT2). Method Firstly, HMGB1 and NFAT2 plasmids were transfected into Hela cells. Doses of HMGB1 plasmid in-creased from 0 to 0.4μg/plate steadily and reporter gene activity of IL-2 was detected and compared. Secondly, endogenous HMGB1 in Hela cells or exogenous HMGB1 in 293T cells was inhibited respectively by sRNAi. Doses of sRNAi for HMGB1 was arisen from 0 to 1.2μg,/plate steadily and reporter gene activity of IL-2 was detected and compared. Results Reporter gene activity of IL-2 increased by 2.12-fold induced by increasing transfection-dose of HMGB1 plasmid in Hela cells (P < 0.01). However, reporter gene activity of IL-2 decreased by 1.7-fold and 4.76-fold repectively due to specific inhibition of exogenous HMGB1 in 293T ceils and endogenous HMGB1 in Hela cells by increasing dose of sRNAi plasmid (P < 0.05 or P < 0.01). Conclusions HMGB1 plays an im-portant role in transcription and expression of IL-2 mediated by NFAT2.