摘要目的 探讨右美托咪定对大鼠胸部撞击性肺损伤的影响.方法 雄性SD大鼠40只,体质量250～300 g,随机(随机数字法)分为五组,每组8只:正常对照组(C);右美托咪定组(D);胸部创伤模型组(T);胸部撞击伤后右美托咪定处理组(TD);胸部撞击模型+右美托咪定+育亨宾组(TDY).各组大鼠6 h后股动脉放血处死,采用酶联免疫吸附实验(ELISA)检测血清中TNF-α和IL1β浓度;检测支气管肺泡灌洗液(BALF)中性粒细胞占白细胞百分比(PMN%);肺湿/干质量比(W/D);HE染色光镜下观察肺组织病理改变.结果 胸部撞击能够导致明显的肺损伤,促使血清TNF-α和IL-1β浓度、支气管肺泡灌洗液W(PMN)%、肺W/D升高(P<O.05,P<0.01),右美托咪定5μg·kg-1·h-1持续静脉输注明显降低胸部撞击所致肺损伤,育亨宾能够部分阻断右美托咪定的这种保护效应.结论 右美托咪定对胸部撞击致急性肺损伤有一定的保护作用.

abstractsObjective To investigate the effect of dexmedetomidine on the outcome of rats with acute lung injury following blunt chest trauma.Method Forty male SD rats weighing 250～300 g were randomly (random number)divided into 5 groups(n=8 each),namely,group C(normal),group D(normal plus dexmedetomidine),group T(trauma),group TD(trauma plus dexmedetomidine),group TDY(trauma plus dexmedetomidine plus yohimbine).Au rats were sacrificed by using exsanguination from arteria femoralis 6 hours later.The TNF-α and IL-1β levels in plasma were examined by using ELISA.Lung wet/dry(W/D)weight and the percentage of neutrophil in leucocytes in bronehoalveolar lavage fluid(BALF)of rats were detected.HE staining was performed to observe the injury of lung tissue under microscope.Results There was significant lung injury after blunt chest trauma.The serum concentrations of TNF-α and IL-1β,PMN%and lung wet/dry(W/D)weight were significantly higher in traumatic group(P<0.05,P<0.01).chest trauma,but this protective effect of dexmedetomidine could be blocked by yohimbine,at least in part,via the inhibition of α2-adrenergie receptor.Conclusions Dexmedetomidine has a certain protective effect on acute traumatic acute lung injury in rats.