摘要目的 观察创伤脓毒症患者血清小分子蛋白组学特征,并观察患者血清小分子蛋白质组随脓毒症进程的变化规律.方法 基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption/ionization time of flight mass spectrometry,MALDI-TOF-MS)技术对血清样本进行蛋白质谱分析,比较24例见刊人、24例创伤患者、12例创伤后脓毒症患者的血清小分子蛋白水平差异,经聚类分析,揭示创伤脓毒症患者的蛋白组学特性.通过对创伤脓毒症患者发病过程中血清小分子蛋白水平的连续观察,通过聚类分析,揭示创伤脓毒症发病进程的组学特征.结果 创伤脓毒症患者血清小分子蛋白谱与创伤对照组和健康对照组比较,均存在明显差异,发现了28个差异蛋白分子,处在不同创伤脓毒症发展阶段的患者的血清小分子蛋白谱之间也存在着明显差别.结论 血清小分子蛋白谱的波动可以较早诊断创伤脓毒症,并可通过血清小分子蛋白谱的变化观察创伤脓毒症的严重程度.

abstractsObjective To observe the features of the serum low molecular weight proteome in pateints subjected to posttraumatic sepsis.Methods Serum proteomic spectra were generated by matrixassisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) technology.Serum samples of 24 healthy controls,24 traumatic patients and 12 posttraumatic sepsis patients were analyzed by comparing the level of low molecular weight proteins.And hierarchical cluster analysis was used to reveal the group characteristics in the process of the pathogenesis of posttraumatic sepsis by continuous observation of the serum level of low molecular weight proteins.And hierarchical cluster analysis was used to reveal the group characteristics in the process of the pathogenesis of posttraumatic spesis by continuous boservation of the serum level of low molecular weight proteome.Results Comparing peak intensities of serum from traumatic patients,posttraumatic sepsis patients with those from healthy controls,mean intensity differed significantly for 28 peaks.And there is a clear difference in the serum low molecular weight proteome profiling of patients between different stages of sepsis development.Conclusions The spectrum of the fluctuations in the serum low molecular weight proteome can be used for earlier diagnosis of sepsis,and severity of sepsis can be predicted by observing the changes of serum low molecular weight proteome profiling.