摘要目的 评估尿液中的中性粒细胞明胶酶相关脂质运载蛋白(uNGAL)对监护病房(ICU)中危重病患者急性肾损伤(AKI)的预测价值.方法 研究对象是分别来自三家综合性医院ICU的110例患者.患者均为18岁以上成年人,入ICU后连续观察72 h,根据AKI诊断的REFLE标准将患者分为AKI组33例和非AKI组77例.根据脓毒症诊断标准将患者又分为脓毒症组79例和非脓毒症组31例.排除标准为慢性肾功能不全、恶性肿瘤、入ICU 24 h内死亡患者.每天检测患者的血肌酐和uNGAL.比较分析脓毒症与非脓毒症患者、AKI与非AKI患者、脓毒症合并AKI与脓毒症非AKI患者之间uNGAL的差异,以及AKI与非AKI患者入ICU第24小时的血肌酐和uNGAL之间的差异,应用ROC曲线评价uNGAL和血肌酐对ICU患者AKI诊断的敏感性和特异性.结果 脓毒症与非脓毒症患者之间,AKI与非AKI患者之间,脓毒症合并AKI与脓毒症非AKI患者之间的uNGAL质量浓度差异具有统计学意义.在入ICU第24小时AKI与非AKI患者uNGAL质量浓度差异具有统计学意义,血肌酐浓度差异无统计学意义.患者入ICU第24小时的uNGAL和血肌酐的ROC曲线下面积分别为0.828 (95％CI:0.742～0.914)和0.583 (95％CI:0.471～0.695).uNGAL的cutoff值为170 ng/ml,敏感性和特异性分别为0.778和0.784,敏感性优于血肌酐.结论 uNGAL在诊断AKI方面优于血肌酐,能够作为ICU患者AKI早期诊断的标志物.

abstractsObjective To estimate the predictive value of neutrophil gelatinase-associated lipocalin in urine (uNGAL) for detection of acute kidney injury (AKI) in the intensive care unit (ICU) critically ill patients.Methods A total of 110 patients from the ICU of three general hospitals were enrolled in the study.The patients were adults more than 18 years of age.After admitted to ICU,the patients were continuously observed for 72 hours.According to the RIFLE criteria for diagnosis of AKI,the patients were classified as AKI group (33 cases) or non-AKI (77 cases).According to the sepsis diagnostic criteria,the patients were classified as sepsis (79 cases) or non-sepsis (31 cases).Exclusion criteria of patients were chronic renal insufficiency,malignant tumor,death after admitted to ICU 24 hours.Serum creatinine and uNGAL of the patients were analyzed daily.The difference of uNGAL between sepsis and non-sepsis patients,AKI and non-AKI patients,sepsis non-AKI and sepsis AKI patients was compared.Moreover,the difference of serum creatinine and uNGAL between AKI and non-AKI patients into ICU 24 h was compared,and the sensitivity and specificity of uNGAL and serum creatinine for diagnosis of AKI in the ICU patients were evaluated using ROC curve.Results The uNGAL levels were all significantly different between sepsis and non-septis patients,AKI and non-AKI patients,sepsis concomitant AKI and sepsis without AKI patients.The uNGAL levels were significantly different between AKI and non-AKI patients in ICU for the first 24 h,while the difference of serum creatinine were not significant.The area under receiver operating characteristic (ROC) curve of uNGAL and serum creatinine of patients in ICU for the first 24 h were 0.828 (95％ CI:O.742-0.914) and 0.583 (95％ CI:0.471-0.695),respectively.The cutoff value of uNGAL was 170 ng/ml,and the sensitivity and specificity were 0.778 and 0.784,respectively.The sensitivity was superior to serum creatinine.Conclusions uNGAL was superior to serum creatinine in the diagnosis of AKI,and could be used as a marker of the early diagnosis of AKI.