摘要目的:探讨丙酮酸乙酯（EP）对热打击后血管内皮细胞增殖活性的影响。方法:将人脐静脉血管内皮细胞（HUVECs）分别置入不同温度设置（39℃，41℃，43℃）的细胞培养箱内进行热打击4 h，或放入相同温度设置（43℃）的细胞培养箱内接受热打击不同时间（2 h，3 h，4 h）（时间点选取的依据——以上时间点是根据预实验的结果来选择的，预实验中43℃持续热打击5 h时细胞大部分都出现坏死脱壁，崩解成细胞碎片），对照组（CONT）则始终置于37℃细胞培养箱中培养，然后在倒置显微镜下观察各组细胞的形态学变化，并采用CCK-8试剂盒检测各组细胞的增殖活性；根据上述实验结果选取合适的热打击温度（43℃）及时间点（4 h），予以浓度为10 mmol/L的EP进行干预，然后采用CCK-8试剂盒检测细胞的增殖活性。结果:随着热打击温度不断升高或热打击时间不断延长，镜下细胞的形态逐渐发生变化，以43℃热打击4 h组(HS组）细胞的形态变化最为明显；另外随着热打击温度的不断升高和热打击时间的不断延长，细胞的增殖活性逐渐降低，且与对照组（CONT）相比，均以43℃热打击4 h组细胞的增殖活性降低最为显著（ F=25.79， P<0.001），而且与热打击组（HS）相比，EP干预组（HS+EP）细胞的增殖活性明显增高（ P<0.001）。 结论:EP可以明显减轻热打击对HUVECs增殖活性的影响，有助于缓解高热所造成的血管内皮细胞活性的改变。

abstractsObjective:To investigate the effect of ethyl pyruvate on proliferation activity of vascular endothelial cells after heat stress.Methods:Human umbilical vein endothelial cells (HUVECs) were placed in incubators with different temperatures (39 ℃, 41 ℃, 43 ℃) for heat shock for 4 h, or placed into the incubator with the same temperature at 43 ℃ and received heat shock for different times (2 h, 3 h, 4 h), and the control group were always placed in 37 ℃ incubator. Then the morphological changes of the cells were observed under an inverted microscope, and the cell proliferation activity was detected by the cell counting - 8 (CCK-8) kit. According to the above experimental results, the optimal intervention temperature (43 ℃) and time point (4 h) of heat stress were selected. After that, ethyl pyruvate (EP) with a concentration of 10 μmol/L was used for intervention (HS+EP group), and the cell proliferation activity was detected by CCK-8.Results:With the increasing of the heat stress temperature or the extension of exposure time, the cell morphology gradually changed under the inverted microscope, and the cells in 43 ℃ incubator for 4 h was the most obvious; and the cell proliferation activity of HUVECs decreased gradually, and the most significant decrease occurred in the group that exposure for 4 h in 43 ℃ ( F=25.79, P < 0.001 vs. control group). In addition, the cell proliferation activity of HUVECs in the HS+EP group was significantly higher than that in the HS group ( P < 0.001). Conclusions:EP can reduce significantly the effect of heat stress on the proliferation activity of HUVECs, and help to alleviate the changes of vascular endothelial cell activity caused by heat stress.