昆虫病原真菌与硅藻土混用对四纹豆象的毒力效果

Efficacy of entomopathogenic fungi in combination with diatomaceous earth against Callosobruchus maculatus(Coleoptera: Bruchidae)

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摘要在室内条件下检测了白僵菌Beauveria bassiana (Balsamo) Vuillemin菌株IRAN-187C和金龟子绿僵菌Metarhizium anisopliae (Metschnikow) Sorokin菌株A-396分别与硅藻土(DE)制剂InsectoSec(R)混用对四纹豆象Callosobruchus maculatus成虫的毒力效果.结果表明:硅藻土与致死剂量和亚致死剂量的真菌分离物混用导致了四纹豆象成虫的高死亡率,且这些处理均缩短了真菌的致死中时(LT50).LC50的白僵菌B.bassiana菌株IRAN-187C和LC50的DE混用对四纹豆象的LT50 (122.2 h)最低,在95％置信限上与LC50的金龟子绿僵菌M.anisopliae菌株A-396与LC50的DE混用得到的LT50(128.2 h)相当,说明两种真菌分离物与DE混用对四纹豆象成虫都具有较好的毒力效果.用四纹豆象成虫检测3个温度(22,27和30℃)和2个相对湿度(40％和55％)组合对DE活性的影响,结果显示30℃和40％相对湿度条件下得到的LT50(110.11 h)最低,说明高温低湿条件下DE活性较高.

作者 Neda NABAEI ^[1] Ali MEHRVAR ^[2] Moosa SABER ^[1] Mohammad BAGHERI ^[1] 学术成果认领

作者单位 Department of Plant Protection, Faculty of Agriculture, University of Maragheh, 55181-83111, Maragheh, Iran ^[1] Department of Plant Protection, Faculty of Agriculture, Azarbaijan Shahid Madani University, 53751-71379, Tabriz, Iran ^[2]