摘要Background Hypertrophic obstructive cardiomyopathy(HOCM)is one of the main reasons for sudden cardiac death(SCD)in young people.Researches has revealed that immune-related genes are closely relevant to HOC-Mprogression.Therefore,it is important to explore the key immuneregulatory mechanisms and biomarkersof HOCM progression.Methods The bioinformatics methods,including linear models for microarray analysis(LIMMA),protein-protein interaction(PPI)network,Gene Ontology(GO),Kyoto Encyclopedia of Genes and Ge-nomes pathway(KEGG)and CIBERSORT,were used to assess the key pathways and hub genes involved in HOCM.Furthermore,expression levels of hub genes were validatedin human tissue.Results Our results showed that the degree of infiltration of five immune cells was linked to HOCM progression,including monocytes,macro-phages M2,natural killer(NK)cell resting,B cells native,and T cells regulatory(Tregs).A total of 7 hub genes(CCL2,CXCL8,FOS,MAP2K1,NFKBIA,STAT3,and TNFRSF1A)were identified and validated by quantitative real-time polymerase chain reaction(qt-PCR).The core genes including CCL2,MAP2K1,NFKBIA,STAT3,and TNFRSF1A are closely related to monocytes infiltration during HOCM progression.Conclusions Taken togeth-er,our research provided useful information to explore the immune mechanisms underlying HCM progression and to provide a potential therapeutic target for therapy in HOCM.The interactional relationship of GATA5,BCL3,and ATF1 complex-regulation CCL2,MAP2K1,NFKBIA,STAT3,and TNFRSF1A was involved in the regulation of monocytes tissue infiltration,which was closely related to the progression of HOCM.[S Chin J Cardiol 2024;25(4):260-274]