摘要目的 探讨乳腺浸润性导管癌中增殖细胞核抗原( proliferative cell nuclear antigen,PCNA)与错配修复基因hMSH2表达的关系及意义.方法 利用组织芯片通过免疫组织化学方法检测68例乳腺癌切除石蜡标本中PCNA和hMSH2的表达.结果 68例乳腺癌组织中,PCNA的阳性表达率为44.1％( 30/68),hMSH2的阳性表达率为54.4％ (37/68).PCNA和hMSH2表达均与淋巴结转移状态相关(P＜0.05).PCNA和hMSH2表达呈正相关(r=0.278,P=0.022).结论 PCNA与hMSH2表达的相互影响与乳腺癌的发生可能有关,联合检测PCNA和hMSH2蛋白有助于判断乳腺癌的恶性程度和生物学行为.

abstractsObjective To explore the relationship between the expression of human proliferating cell nuclear antigen (PCNA) and human MutS honolog2 gene (hMSH2) in breast cancer and its significance. Methods PCNA and hMSH2 expression were detected in 68 cases of breast cancer tissues by immunohistochemistry.Results Among the 68 cases of breast cancer, the expression rate of PCNA and hMSH2 was 44.1％ (30/68) and 54.4％ (37/68) respectively.The expression of PCNA and hMSH2 was positively correlated with lymph nodes metastasis(P ＜0.05).PCNA expression was positively correlated to hMSH2 expression in breast cancer (P ＜0.05).Conclusion Interaction between PCNA and hMSH2 may be related to the carcinogenesis of breast cancer.Effective detection of PCNA and hMSH2 proteins may contribute to the evaluation of malignancy and biological behavior of breast cancer.