摘要目的:探究LncRNA抗分化非编码RNA（anti-differetiation non-coding RNA, ANCR）在胃癌患者肿瘤组织中表达的临床意义及其对细胞的生物学效应。方法:收集宁波医疗中心李惠利医院东部院区2016年9月至2018年6月胃癌组织及相应癌旁组织标本各72例，同时培养胃癌细胞HGC-27，慢病毒转染ANCR cDNA全长载体于HGC-27细胞中作为实验组，并转染空白载体作为对照组；实时荧光定量PCR（qPCR）技术检测组织或细胞中ANCR、转录因子Oct4及Sox2 mRNA的表达水平，免疫印迹实验（western blot）检测细胞中Oct4及Sox2蛋白表达水平，CCK-8实验检测两组细胞增殖能力，Transwell侵袭及迁移实验检测两组细胞转移能力。结果:胃癌组织及癌旁组织中ANCR表达分别为0.013（0.006，0.025）及0.041（0.011，0.136），胃癌组织中ANCR表达显著高于癌旁组织（ P<0.01），且高表达ANCR的患者TNM分期更高及细胞分化程度更低（ χ2=7.414、8.236， P<0.05）。对照组及实验组细胞ANCR mRNA表达分别为（1.000±0.064）、（6.250±0.889），Oct4 mRNA表达分别为（1.000±0.208）、（2.815±0.349），Sox2 mRNA表达分别为（1.000±0.173）、（2.526±0.390），Oct4蛋白表达分别为（1.000±0.148）、（3.396±0.105），Sox2蛋白表达分别为（1.000±0.119）、（2.916±0.130），实验组细胞ANCR、Oct4、Sox2 mRNA表达显著高于对照组（ P<0.01），实验组细胞Oct4、Sox2蛋白表达水平明显高于对照组（ P<0.01）。对照组及实验组细胞72h的相对增殖能力分别为（7.164±0.426）、（9.627±0.605）；96h的相对增殖能力分别为（13.750±1.089）、（19.166±1.649），实验组细胞72 h、96 h的增殖能力显著高于对照组（ P<0.01）。对照组及实验组每视野平均侵袭细胞数分别为（17.26±5.48）个、（39.43±5.21）个；迁移细胞数分别为（30.49±7.74）、（62.20±7.51）个，实验组迁移及侵袭细胞数显著多于对照组（ P<0.01）。 结论:LncRNA ANCR在胃癌患者肿瘤组织中表达显著增高，且与患者病情进展及细胞恶性程度密切相关，体外可促进胃癌干细胞标志物的表达，并增强细胞增殖及转移能力。

abstractsObjective:To investigate the clinical significance of LncRNA anti-differetiation non-coding RNA (ANCR) expression in tumor tissues of gastric cancer patients and its biological effects on cells.Methods:72 cases of gastric cancer tissues and corresponding adjacent tissues were collected from Sep. 2016 to Jun. 2018 in our Hospital. Gastric cancer cell HGC-27 was cultured, lentiviral transfected ANCR cDNA full-length vector was used as a Test group in HGC-27 cells, and transfected blank vector as a control group. Real-time quantitative PCR (qPCR) was used to detect the expression of ANCR, transcription factor Oct4 and Sox2 mRNA in tissues or cells, Western blot was used to detect the expression levels of Oct4 and Sox2 in cells, CCK-8 assay was employed for detecting cell proliferation in both groups, and Transwell invasion and migration assay was used to detect the transfer ability of cells in the two groups.Results:The expressions of ANCR in gastric cancer and corresponding adjacent tissues were respectively 0.013 (0.006, 0.025) and 0.041 (0.011, 0.136) , and the expression of ANCR in gastric cancer tissues was significantly higher than that in adjacent tissues ( P<0.01) , and patients with high expression of ANCR had higher TNM stage and lower cell differentiation ( χ2=7.414 and 8.236, P<0.05) . The expressions of ANCR mRNA in control group and test group were respectively 1.000±0.064 and 6.250±0.889, Oct4 mRNA were respectively 1.000±0.208 and 2.815±0.349, Sox2 mRNA were respectively 1.000±0.173 and 2.526±0.390, Oct4 protein were respectively 1.000±0.148 and 3.396±0.105, Sox2 protein were respectively 1.000±0.119 and 2.916±0.130, and the expressions of ANCR, Oct4 and Sox2 mRNA in the test group were significantly higher than those in the control group ( P<0.01) ; the expression levels of Oct4 and Sox2 protein in the test group were significantly higher than those in the control group. The proliferation abilities of control group and test group were 7.164±0.426 and 9.627±0.605 in 72h, and 13.750±1.089 and 19.166±1.649 in 96h. The proliferation of cells in the Test group at 72 and 96 hours was significantly higher than that in the control group ( P<0.01) . The average number of invasive cells per visual field in control group and test group were 17.26±5.48 and 39.43±5.21, and number of migration cells were 30.49±7.74 and 62.20±7.51, and the number of migration and invasion cells in the Test group was significantly larger than that in the control group ( P<0.01) . Conclusions:The expression of LncRNA ANCR in tumor tissues of gastric cancer patients is significantly increased, and it is closely related to the progression of the disease of patients and the degree of cell malignancy. It can promote the expression of gastric cancer stem cell markers in vitro and enhance the ability of cell proliferation and metastasis.