摘要目的:分析妊娠期糖尿病（gestational diabeies mellitus，GDM）孕妇脂肪组织及胎盘组织中过氧化物酶体增殖物激活受体（PPARγ）mRNA和蛋白相对表达量。方法:选取2019年1月至2020年11月烟台市烟台山医院妇产科住院待产的GDM孕妇52例（观察组）与同时期门诊体检并分娩的正常孕妇50例（对照组），采集血清标本后检测胰岛细胞功能。两组孕妇分娩后采集其胎盘组织标本，测定胎盘组织与脂肪组织的PPARγ mRNA及PPARγ蛋白含量，并分析GDM孕妇胎盘、脂肪组织PPARγ mRNA及PPARγ蛋白与胰岛细胞功能的相关性。结果:观察组HOMA-IR水平高于对照组[（3.45±1.06）% vs（1.40±0.43）%]、HOMA-β水平[（126.59±23.59）% vs（153.12±27.34）%]低于对照组（ P<0.05）。观察组胎盘组织PPARγ mRNA[（1.65±0.21）vs（0.93±0.16）]与PPARγ蛋白含量[（1.89±0.51）vs（1.02±0.23）]高于对照组（ P<0.05），脂肪组织中PPARγmRNA[（0.49±0.12）vs（1.15±0.26）]及PPARγ蛋白含量[（0.43±0.11）% vs（0.96±0.22）%]低于对照组（ P<0.05）。脂肪组织PPARγ mRNA及PPARγ蛋白与HOMA-IR（ r=-0.45，-0.33）呈负相关性，与HOMA-β（ r=0.47，0.43）呈正相关性（ P<0.05）；胎盘组织PPARγ mRNA及PPARγ蛋白与HOMA-IR（ r=0.40，0.37）呈正相关性，与HOMA-β（ r=-0.44，-0.35）呈负相关性（ P<0.05）。 结论:PPARγ mRNA与PPARγ蛋白水平在GDM患者脂肪组织中呈低表达，在胎盘组织中反馈性高表达，且PPARγ表达与HOMA-IR、HOMA-β均有明显相关性，可为GDM的临床治疗提供新靶点和方向。

abstractsObjective:To analyze the clinical value of relative expression of peroxisome proliferators-activated receptors mRNA (PPAR mRNA) and protein in placental tissues of pregnant women with gestational diabetes mellitus (GDM) .Methods:52 pregnant women (the study group) with GDM who were admitted to our hospital from Jan. 2019 to Nov. 2020 and 50 normal pregnant women (the control group) who underwent physical examination and gave birth during the same period were selected. Serum samples were collected to detect the islet cell function. Placental tissue samples of the two groups of pregnant women were collected after delivery to measure the content of PPARγmRNA and protein in placental tissues and adipose tissues. The correlations between PPARγ in placental, adipose tissues of GDM pregnant women with islet cell function were analyzed.Results:The HOMA-IR level of the study group was higher than that of the control group [ (3.45±1.06) % vs (1.40±0.43) %], and the HOMA-β level [ (126.59±23.59) % vs (153.12±27.34) %] was lower than that of the control group ( P<0.05). The PPARγ mRNA [ (1.65±0.21) vs (0.93±0.16) ] and PPARγ protein content [ (1.89±0.51) vs (1.02±0.23) ] of placenta tissue in the study group were higher than those in the control group ( P<0.05), the PPARγmRNA [ (0.49±0.12) vs (1.15±0.26) ] and PPARγ protein content [ (0.43±0.11) % vs (0.96±0.22) %] in adipose tissue were lower than those of the control group ( P<0.05). Adipose tissue PPARγ mRNA and PPARγ protein were negatively correlated with HOMA-IR ( r=-0.45, -0.33), and positively correlated with HOMA-β ( r=0.47, 0.43) ( P<0.05) ; placental tissue PPARγ mRNA and PPARγ protein were positively correlated with HOMA-IR ( r=0.40, 0.37), and negatively correlated with HOMA-β ( r=-0.44, -0.35) ( P<0.05) . Conclusion:The levels of PPARγ mRNA and PPARγ protein are low expressed in adipose tissue of GDM patients, and highly expressed in placental tissues, and PPARγ expression is significantly correlated with HOMA-IR and HOMA-β, which can provide new clinical treatment for GDM Target and direction.