摘要Colorectal cancer(CRC)is one of the most common malignancies worldwide.Development of predictive molecular markers may help to achieve the best outcome in clinic.The purpose of this study is to identify the differentially expressed genes and new potential predictive and prognostic molecular biomarkers for CRC.In this study,CRC and matched normal tissues acquired from the same patient were used to extract total RNA.The NanoString nCounter assay was applied to determine the differentially expressed genes.The results were then validated by using the Cancer Genome Atlas data.Finally,we identified 27 genes that revealed significant correlation with CRC in the tumor tissue.Several genes in the pan-cancer panel showed significant differential expression,which were more universal than others in the CRC tissue.Since some of them have not been reported as being directly related to CRC yet,future mechanism studies can be designed based on this study.Our study demonstrated NanoString nCounter assay could serve as an alternative approach for gene expression analysis and identified several unreported differently expressed genes in CRC patients,which may provide some important clues for more in-depth study of CRC and serve as potential predictive molecular biomarkers for clinical diagnosis application.