摘要A rapid method was presented for the determination of 19 polyphenols in tea by ultra-high performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry(UPLC-Q-TOF MS).Tea samples were extracted by 50％(V/V)ethanol,then separated by Waters Acquity BEH C18 column using a binary solvent system composed of acetonitrile and water(0.1％formic acid)by gradient elution.The analytes were determined by Q-TOF MS in TOF MS and information dependent acquisition(IDA)-MS/MS mode.The results showed that mass accuracy error of the 19 polyphenols were lower than 5.0×10-6,good linear relationship was got in range of 0.2-500 μg/L and correlation coefficient was higher than 0.999 0.The LOD was in the range of 0.002-0.100 mg/kg and the LOQ was in the range of 0.004-0.200 mg/kg.Recovery of the method was in range of 78.4％-109.2％with spike levels of 0.004-2.000 mg/kg,relative standard deviations were lower than 10％.The method was simple,rapid and accurate.It could be used for the rapid screening and quantitative analysis of 19 polyphenols in tea.