摘要Mitochondrial dysfunction is proposed to be substantially associated with ageing and ageing-related diseases like Alzheimer's disease(AD).However,it is unclear whether different mouse models with mitochondrial-related diseases have similar changes in mitochondrial morphology of the same tissues.Moreover,whether similarities in mitochondrial morphology can be a suitable marker for screening and/or discovering mitochondrial-protective substances remains unknown.Mitochondria morphology in different tissues of a novel mitochondrial outer membrane protein Slc25a46 knockout mouse and a traditional APPswE/PS1ΔE9 transgenic mouse were examined using transmission electron microscope(TEM).Both young Slc25a46 knockout mice and aged APPswE/PS1ΔE9 mice models showed similar mitochondrial damage in cerebellum tissues.The results indicated that different mitochondrial-related diseases shared similar alteration and defects in mitochondrial morphology.Furthermore,Lycium ruthenicum Murr.extract,a bioactive food substance with cognition-improving property,could effectively improve muscle strength and increase body weight in the Slc25a46 knockout mice.These findings suggest that mitochondrial morphology defects in mice models,particularly in the mitochondrial compartment,represent a unified and effective marker for screening and validating natural product-derived functional substances with mitochondrial protective properties.It also holds potential application in mitochondrial-impaired senile neurodegenerative diseases,especially in AD.