摘要7-Ketophytosterols are the major oxidation products of phytosterols in foods,which have been associated with atherosclerosis.However,their absorption mechanism remains unclear.The aim of our work was to investigate the absorption mechanism of 7-ketophytosterols and their effects on the cholesterol transport using Caco-2 cell model.The absorption percentage of 7-ketositosterol and 7-ketocampesterol was 1.16％-1.68％and 1.18％-2.23％respectively in the Caco-2 model,which is higher than that of their parent phytosterols,but lower than cholesterol-d7.The apparent permeability of 7-ketositosterol and 7-ketocampesterol at 30 μmol/L in the basolateral(BL)-to-apical(AP)direction were 0.42-and 0.55-fold of that in the AP-to-BL direction,indicating an active intake in the permeation mechanism of 7-ketophytosterols.Ezetimibe could significantly inhibit the transport of 7-ketophytosterols(P＜0.05),which means that their transport depends on niemann-pick cl-like 1(NPC1L1)protein.The transport of cholesterol-d7 was significantly inhibited by 7-ketophytosterols(P＜0.05).Taken together,this study deepened our understanding of the absorption mechanism of common food-born 7-ketophytosterols and provides useful information on the inhibition of 7-ketophytosterols absorption.