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Exploration of cyclooxygenase-2 inhibitory peptides from walnut dreg proteins based on in silico and in vitro analysis

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摘要Walnut dreg protein hydrolysates(WDPHs)exhibit a variety of biological activities,however,the cyclooxygenase-2(COX-2)inhibitory peptide of WDPHs remain unclear.The aim of this study was to rapidly screen for such peptides in WDPHs through a combination of in silico and in vitro analysis.In total,1 262 peptide sequences were observed by nano liquid chromatography/tandem mass spectrometry(nano LC-MS/MS)and 4 novel COX-2 inhibitory peptides(AGFP,FPGA,LFPD,and VGFP)were identified.Enzyme kinetic data indicated that AGFP,FPGA,and LFPD displayed mixed-type COX-2 inhibition,whereas VGFP was a non-competitive inhibitor.This is mainly because the peptides form hydrogen bonds and hydrophobic interactions with residues in the COX-2 active site.These results demonstrate that computer analysis combined with in vitro evaluation allows for rapid screening of COX-2 inhibitory peptides in walnut protein dregs.

作者 Zishan Hong ^[1] Jing Xie ^[1] Liang Tao ^[1] Jing-Jing Dai ^[2] Tingting Li ^[3] Li Zhang ^[4] Yuying Bai ^[3] Xia Hu ^[4] Jinlian Chen ^[3] Jun Sheng ^[5] Yang Tian ^[6] 学术成果认领

作者单位 College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Yunnan Provincial Key Laboratory of Precision Nutrition and Personalized Food Manufacturing,Yunnan Agricultural University,Kunming 650201,China;Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources,Ministry of Education,Yunnan Agricultural University,Kunming 650201,China ^[1] Yunnan Provincial Key Laboratory of Precision Nutrition and Personalized Food Manufacturing,Yunnan Agricultural University,Kunming 650201,China;School of Tea and Coffee,Pu'er University,Pu'er 665000,China;Yunnan Provincial Engineering Research Center for Edible and Medicinal Homologous Functional Food,Yunnan Agricultural University,Kunming 650201,China ^[2] College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Yunnan Provincial Key Laboratory of Precision Nutrition and Personalized Food Manufacturing,Yunnan Agricultural University,Kunming 650201,China;Yunnan Provincial Engineering Research Center for Edible and Medicinal Homologous Functional Food,Yunnan Agricultural University,Kunming 650201,China ^[3] College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources,Ministry of Education,Yunnan Agricultural University,Kunming 650201,China;Yunnan Provincial Engineering Research Center for Edible and Medicinal Homologous Functional Food,Yunnan Agricultural University,Kunming 650201,China ^[4] College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Key Laboratory of Pu-er Tea Science,Ministry of Education,Yunnan Agricultural University,Kunming 650201,China ^[5] College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Yunnan Provincial Key Laboratory of Precision Nutrition and Personalized Food Manufacturing,Yunnan Agricultural University,Kunming 650201,China;Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources,Ministry of Education,Yunnan Agricultural University,Kunming 650201,China;School of Tea and Coffee,Pu'er University,Pu'er 665000,China;Yunnan Provincial Engineering Research Center for Edible and Medicinal Homologous Functional Food,Yunnan Agricultural University,Kunming 650201,China ^[6]

关键词 Walnut dreg proteins Cyclooxygenase-2 inhibitory peptide Identification Virtual screening Molecular docking

DOI 10.26599/FSHW.2022.9250143

发布时间 2024-05-20（万方平台首次上网日期，不代表论文的发表时间）

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