摘要This study demonstrates the feasibility of producing three polysaccharides(neutral LJP-1,acidic LJP-2 and acidic LJP-3)with significant in vitro and in vivo anti-inflammatory activities from the flowers of Lonicera japonica.The three polysaccharides differed in chemical composition,molecular weight(Mw)distribution,glycosidic linkage pattern,functional groups and morphology.They exhibited excellent protective effects(in a dose-dependent manner)in lipopolysaccharide-injured RAW264.7 macrophages and CuSO4-damaged zebrafish via reducing NO production and inhibiting the overexpressions of inflammation-related transcription factors,inflammatory proteins and cytokines in the NF-κB/MAPK signaling pathways.Their anti-inflammatory effects varied owing to their different molecular characteristics and chemical compositions.Overall,LJP-2 at 400 μg/mL was the most effective.LJP-2 consisted mainly of →5)-α-L-Araf(1→,→4)-α-L-GalpA(1→ and →2)-α-L-Rhap(1→ residues with terminal T-β-D-Glcp.Thus,honeysuckle flowers are good sources of anti-inflammatory polysaccharides,and precise fractionation enables the production of potent anti-inflammatory agents for the development of functional foods and healthcare products.