摘要Melamine is one of the most frequently detected adulterants in dairy products.The current study proposes a surface-enhanced Raman spectroscopy(SERS)-based analytical tool for fast and reliable screening of melamine in bovine milk.A hand-held Raman spectrometer was used in conjunction with a substrate composed of silver nanoparticles(AgNPs)that was 2D printed onto glass fiber(GF)filter paper.Under optimized conditions,a sensitive and fingerprint-like signal at 674 cm-1 was obtained.The AgNPs/GF substrate exhibited high sensitivity to melamine in milk down to 1.949 8×10-5 mg/mL,which is well below the USA and EU safety limits(2.5×10-3 mg/mL).Remarkably,the proposed technology was also highly reproducible,showing spot-to-spot and block-to-block variations below 3.3%and 4.9%at 674 cm-1 in Raman intensity,respectively.The characteristic peak intensity and concentration of melamine showed an acceptable linear relationship(R2=0.990 9)within the range of 0.000 1-1 mg/mL.Overall,the method established in this study can provide an efficient and effective method for the quantitative target screening and detection of melamine in dairy products.