摘要A tri-signal ultrasensitive colorimetric/electrochemical detection of ovomucoid(OM)was developed.Herein,copper oxide nanoparticles(CuO NPs)were prepared,which exhibit excellent enzyme-like activity(peroxidase-like and laccase-like)and electrochemical activity.CuO@3-APBA nanoparticles(CuO@3-APBA NPs)were prepared by the coordinating Cu with the amino group on 3-aminophenobenic boric acid(3-APBA)in CuO NPs.3-APBA of CuO@3-APBA can react with diol structure on sugar chain of OM under alkaline conditions.Then,a tri-signal ultrasensitive biosensing platform for OM was established based on the catalytic activity of CuO@3-APBA nanozyme.For the first signal,CuO@3-APBA can catalyze oxidation of 1,3,5-trimethylbenzene(TMB)to turn the solution from colorless to blue in the presence of H2O2(absorbance at 652 nm).For the second signal,CuO@3-APBA can catalyze the oxidation of substrates(2,4-dichlorophenol and 4-aminoantipyrine)and turn the solution from colorless to pink(absorbance at 510 nm).For the third signal,electrochemical oxidation peak of copper ion from Cu+to Cu2+of CuO@3-APBA was recorded by differential pulse voltammetry,which was used to determine the OM.The sensing platform exhibited a wide linear range(0.000 031 6-100.000 000 ng/mL)with a low detection limit(0.010 5 pg/mL),as well as showed advantages,such as satisfactory reproducibility,good stability,and excellent selectivity.The assay has the potential applications for ultrasensitive detection of allergen in foods.