摘要Camellia oil,which contains a high content of triterpene alcohol,is known for a series of bioactivities including anti-inflammation.Amyrins are recognized as high bioactivity of anti-inflammation.However,no comparative study on triterpene alcohols from camellia oil.In this study,four high content triterpene alcohols from camellia oil,namely β-amyrin,ψ-taraxasterol,parkeol,and butyrospermol were evaluated through lipopolysaccharide induced RAW264.7 cell inflammation.The results showed that butyrospermol exhibited the highest anti-inflammatory activity,surpassing that of β-amyrin.Characterization of signaling pathways showed that butyrospermol inhibited Toll-like receptor 4(TLR4),nuclear factor κB(NF-κB)and mitogen-activated protein kinase(MAPK)pathways,suppressing the transcription of Tlr4,expression of p65,NF-κB inhibitor α(IκBα),extracellular signal-regulated kinase(ERK),c-Jun N-terminal kinase(JNK),and p38,and the phosphorylation of p65,IκBα,ERK,and p38.The anti-inflammatory effect of butyrospermol was further validated by phorbol 12-myristate 13-acetate induced mouse ear edema.The results in mouse showed that butyrospermol could inhibit the increase of tumor necrosis factor α(TNF-α),interleukin 1β(IL-1β),p-JNK,p-p38,p-IκBα,and their corresponding mRNA levels.Our study provides new perspective on the anti-inflammatory role of different triterpene alcohols and explaining the bioactivity of camellia oil.