摘要Aflatoxin B1(AFB1)in food is a threaten to food safety and human health.In this study,a colorimetric and fluorescent dual-mode aptamer sensor was constructed for rapid and accurate detection of AFB1 based on polyvinylpyrrolidone-gold-copper bimetallic nanoclusters(PVP-Au/CuNCs)and Mn3O4 nanoparticles(Mn3O4 NPs).In the presence of AFB1,the AFB1 aptamer on the surface of Mn3O4 NPs specifically bound to AFB1.The oxidase-like activity of Mn3O4 NPs was restored and 3,3',5,5'-Otetramethylbenzidine(TMB)was oxidized to oxidized TMB(oxTMB),which quenched the orange fluorescence of the PVP-Au/CuNCs.With AFB1 concentrations increasing,the blue color of oxTMB deepened,the fluorescence quenching efficiency was enhanced.The working range was 0-56 μg/L with a limit of detection(LOD)of 0.36 μg/L(colorimetric),and 2.86-40.00 μg/L with a LOD of 0.29 μg/L(fluorescent).In addition,the established sensor was successfully applied to determine AFB,in milk,rice,oats,and corn,with a recovery rate of 91.1％-107.5％.This study provides an insight for fabricating a sensitive,efficient,and accurate analytical platform for rapid detection of AFB1 in food.