摘要High-fat diets(HFD)disrupt lipid homeostasis,posing major public health risks.This study investigated the effect of ACA-DK,an insoluble dietary fiber derived from Antrodia camphorata,on HFD-induced dyslipidemia.We demonstrate that ACA-DK effectively alleviates HFD-induced dyslipidemia in mice,counteracting metabolic disorders,aberrant blood lipids,and weight gain.Mechanistically,ACA-DK modulates triglycerides,phosphatidylcholine,and phosphatidylethanolamine via glycerophospholipid/choline/linoleic acid metabolism pathways,while rectifying gut dysbiosis through selective reduction of pro-inflammatory genera(Oscillibacter,Ruminiclostridium,Negativibacillus,Ruminococcaceae and Helicobacter).Integrated analysis identifies Ruminococcaceae and Helicobacter as key mediators of AC A-DK's lipid-regulatory effects,establishing microbiota-directed therapy as a strategy against dyslipidemia.We thus propose ACA-DK as a microbiota-directed dual-target therapy,simultaneously reprogramming host lipid metabolism and gut ecology to combat diet-induced metabolic diseases.These findings suggest that ACA-DK is a promising prebiotic dietary fiber for ameliorating HFD-induced lipid metabolic disorders,with potential for future development into functional foods or supplements.