摘要This study aimed to develop a submicron emulsion(SE)of Acer truncatum seed oil(ASO)and Rose roxburghii Tratt juice(RRTJ)using a response surface method for optimal formula screening and process parameters.The stability of ASO-RRTJ/SE and its effects on scopolamine-induced memory impairment in mice were investigated.The ASO-RRTJ/SE exhibited a desirable particle size((276.86±4.61)nm),polydispersity index(PDI,0.22±0.02),centrifugal stability parameter(Ke,0.143±0.004),and Zeta potential((30.57±2.38)mV).Morris water maze test,measurement of acetylcholine(ACh)concentration and acetylcholinesterase(AChE)activity in the hippocampus,superoxide dismutase(SOD)activity and malondialdehyde(MDA)concentration in serum,hematoxylin and eosin(H&E)staining,immunofluorescence staining were adopt to evaluate ASO-RRTJ/SE therapeutic potential in alleviating scopolamine-induced memory impairment in mice.Behavioral tests demonstrated that ASO-RRTJ/SE significantly ameliorated scopolamine-induced spatial learning and memory deficits in mice,suggesting potential neuroprotective effects against scopolamine-mediated central nervous system excitation.Compared to the Model group,the high-dose ASO-RRTJ/SE(H-SE)group displayed a 77.84%increase in hippocampal ACh content,a 46.97%decrease in AChE activity,a 29.48%increase in serum SOD activity,and a 40.15%decrease in MDA content.H&E staining of hippocampal sections revealed that the H-SE group exhibited well-organized hippocampal neurons,with a significantly reversal of nuclear pyknosis,deep staining,and cytoplasmic dissolution.The pyramidal cell layer displayed improved organization,and the intercellular distance returned to normal.Additionally,H-SE treatment significantly reduced the aggregation of phosphorylated Tau protein,increased choline acetyltransferase expression,and promoted brain-derived neurotrophic factor production.In conclusion,ASO-RRTJ/SE ameliorates scopolamine-induced memory impairment in mice.