摘要This study aimed to gain insight into the interaction and metabolic mechanism between Aspergillus flavus U-16 and Saccharomyces cerevisiae HJ during co-fermentation.Despite the suppressed transcriptional activity of SU-16,its metabolites exerted a pivotal regulatory influence.The metabolites produced by SU-16 significantly increased the ethanol content to 17.0％.They also significantly increased the contents of crucial flavor substances,including amino acids(at least 5 times)and esters(up to 1 100 mg/L).SU-16 produced 130 metabolites that may affect flavor compounds,while HJ mainly controlled the synthesis of 161 compounds.Metabolites of SU-16 with different molecular weights were observed to exert differential regulatory effects on the fermentation process and the metabolic pathway of HJ.The small molecule metabolites of SU-16 promoted the growth of HJ significantly.In contrast,its macromolecular metabolites were found to mainly affect the expression of genes related to carbohydrate and amino acid metabolism in the HJ genome.