摘要Aberrant deposition of collagen is associated with cancer development and tissue fibrosis.Proline hydroxylation,catalyzed by collagen prolyl 4-hydroxylases(C-P4Hs),is necessary for collagen maturation and secretion.Here,we try to evaluate the mechanism of the regulation of CHX on collagen maturation.Using pepsin digestion,liquid chromatograph mass spectrometry and gene knockout,we find that treatment of mouse embryonic fibroblasts with cycloheximide(CHX)increases type Ⅰ collagen proline hydroxylation partially via P4HA1 and mainly via P4HA2.Western blot analysis results show that CHX treatment reduces type Ⅰ collagen but does not obviously impact the level of P4HA1/2 protein in the endoplasmic reticulum,which enhances the molar ratio of P4HA1/2 to type Ⅰ collagen,and coimmunoprecipitation results confirm that more P4HA1/2 can bind to each type Ⅰ collagen.Since C-P4Hs possess the capability to hydroxylate proline independent of ascorbate for a few cycles,this enhanced binding between P4HA1/2 and type Ⅰ collagen can partially explain how CHX stimulates type Ⅰ collagen maturation.