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Recent advances on protein engineering for improved stability

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摘要Stability is a crucial factor influencing applicability of industrial biocatalysts,and enzymatic operations in partial organic solvents and higher temperatures are preferable since organic solvents may improve the solubility of the substrates,and the reaction rates are often higher at elevated temperatures.This review aims to summarize recent advances for engineering enzymes to meet the industrial needs for solvent and thermostability and offer insights in the advances in methodologies utilizing B-factors,ancestral reconstructions,or machine learning approaches.

作者 Jinghao Shi ^[1] Bo Yuan ^[2] Hengquan Yang ^[3] Zhoutong Sun ^[2] 学术成果认领

作者单位 School of Chemistry and Chemical Engineering,Shanxi University,030006,Taiyuan,China;Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,32 West 7th Avenue,Tianjin Airport Economic Area,Tianjin,300308,China;Shanxi Key Laboratory of the Green Catalytic Synthesis of Coal-based High Value Chemicals,China ^[1] Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,32 West 7th Avenue,Tianjin Airport Economic Area,Tianjin,300308,China;Key Laboratory of Engineering Biology for Low-Carbon Manufacturing,Tianjin,300308,China ^[2] School of Chemistry and Chemical Engineering,Shanxi University,030006,Taiyuan,China;Shanxi Key Laboratory of the Green Catalytic Synthesis of Coal-based High Value Chemicals,China ^[3]

关键词 Thermostability Biocatalysis Protein engineering Directed evolution B-factor

栏目名称

Review Article

DOI 10.1016/j.bidere.2025.100005

发布时间 2025-05-09（万方平台首次上网日期，不代表论文的发表时间）