摘要The complexity and intricacy of the brain,which is composed of billions of neurons,pose significant challenges to its study.Understanding neural connections and communication at the single-cell level is crucial for unraveling the brain's functions.This study presents a novel strategy that utilizes magnetic nanoparticles(MNPs)and magnetic fields to manipulate neurons,thereby creating customized small-scale neural circuits for studying neural connections.To establish the feasibility of this approach,the effects of MNPs on neurons were initially investigated,demonstrating their low toxicity.Subsequently,a micro-magnet array(MMA)chip was employed to manipulate the neurons,facilitating their precise arrangement on the electrodes.Over several days,the neurons extended their axons and established connections with neighboring cells,forming small-scale circular neural circuits.These artificially engineered circuits offer a simplified and controlled environment for studying neu-ral networks in contrast to naturally occurring biological networks.Furthermore,electrophysiological recordings were con-ducted to investigate the connections between the manipulated neurons.This study introduces a customized small-scale neu-ral circuit platform with electrode-specific recording and stimulating capabilities,enabling the study of neuron-to-neuron in-teractions at the single-cell level.By leveraging MNPs and an MMA chip,this research offers a powerful tool for studying neural connections and advancing our understanding of the brain's intricate workings.