换一批摘要We developed a small-tissue extraction device(sTED),an automated system that integrates 1-min mechanical disso-ciation and enzymatic digestion to extract viable primary cells from ultrasmall tissue samples(5-20 mg)within 10 min.Unlike conventional methods,sTED minimizes cell loss and enhances reproducibility,achieving>90％cell viability in mouse tissues and>60％in human tumors,with 1.5×104-2.5×104 cells/mg yield from mouse liver.Tailored for biop-sies and ultrasmall samples,sTED addresses critical stan-dardization challenges in organoid-based research.
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