摘要Efficient coupling between acoustic fields and fluid microenvironments is crucial for advancing applied physics and micro-fluidic engineering in advanced biomedical,environmental sustainability,and broader industrial applications.Harnessing such interactions for biological processing enables the precise,contactless,and tunable control of cell membrane disruption,facilitating reagent-free,contamination-minimized lysis.However,existing acoustic lysis devices are faced with challenges of limited efficiency and intricate structures.To overcome these limitations,we developed a staggered traveling-surface Ray-leigh acoustic wave(STRAW)microchip for additive-free cell lysis.The device consists of a LiNbO3 substrate patterned with two sets of interdigital transducers and a circular polydimethylsiloxane ring for confining cell suspension.We con-structed a mathematical model for the STRAW-induced mechanical effects and optimized the alignment of interdigital trans-ducers via theoretical modeling and finite-element analysis to maximize torque and acoustic streaming.The proposed STRAW-based platform showed over 95%lysis efficiency within 30 s for MC3T3-E1 mammalian cells,Gram-negative Escherichia coli,and Gram-positive Staphylococcus aureus.Thus,the developed design enables additive-free,structurally straightforward acoustic lysis with demonstrated compatibility across the tested cell types.Beyond basic lysis,this universal platform can be used in point-of-care diagnostics and food and environmental safety monitoring.This work illustrates how fluid structure-wave interactions may inform fluid mechanics and applied physics within a high-performance,low-complexity microfluidic system,paving the way for the widespread integration of STRAW-induced acoustic streaming in diagnostics,industry,and research.