摘要Despite the importance of studying nucleoprotein complexes,no appropriate method for quantifying them is available.Here,a UV absorbance method using the formula"Cmg/mL=1.55A280-0.76A260"were applied to quantify nucleoprotein complexes.After modification using two paired A260 and A280 values,the UV-derived formula-based method could accurately quantify proteins in nucleoprotein complexes.Otherwise,by taking the target protein as a standard,the Bradford assay can accurately quantify pro-teins in nucleoprotein complexes without interference by nucleic acids.The above methods were suc-cessfully applied to measure the concentration of MtuP49-CTG complexes of Mycobacterium tuberculo-sis.In conclusion,both the Bradford assay and the UV-derived formula-based method were appropri-ate for quantifying proteins in nucleoprotein complexes,which may make contributions to explore the interactions between proteins and nucleic acids at the molecular level.