Seeing is believing: observation of migrasomes

导出学术成果认领

摘要Migrasomes are a novel type of cell organelle that form on the retraction fibers at the rear of migrating cells. In recent years, numerous studies have unveiled the mechanisms of migrasome formation and have highlighted significant roles of migrasomes in both physiological and pathological processes. Building upon the strategies outlined in published works and our own research experiences, we have compiled a comprehensive set of protocols for observing migrasomes. These step-by-step instructions encompass various aspects such as cell culture, labeling, imaging, in vitro reconstitution, and statistical analysis. We believe that these protocols serve as a valuable resource for researchers exploring migrasome biology.

作者 Yuwei Huang ^[1] Li Yu ^[2] 学术成果认领

作者单位 1School of Basic Medical Sciences, Translational Medicine Institute, Xi’an Jiaotong University, Xi’an 710049, China ^[1] 2State Key Laboratory of Membrane Biology, Tsinghua University-Peking University Joint Center for Life Sciences, Beijing Frontier Research Center for Biological Structure, School of Life Sciences, Tsinghua University, Beijing 100084, China ^[2]

关键词 Migrasome Labeling Imaging In vitro reconstitution

栏目名称 PROTOCOL

DOI 10.52601/bpr.2023.230024

发布时间 2024-07-26

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