摘要目的 探讨宫内生长受限(IUGR)胎鼠脑组织牛磺酸水平及孕鼠补充牛磺酸对IUGR胎鼠脑组织牛磺酸水平的影响.方法 采用全程低蛋白饮食法建立IUGR胎鼠模型,将15只孕鼠随机分为3组:正常对照组、IUGR组、IUGR+孕鼠补充牛磺酸组(牛磺酸组).牛磺酸组自怀孕第12天起饮食中添加牛磺酸300 mg/(kg·d)直至自然分娩.每窝随机抽取2只胎鼠断头取脑,采用液质联用串联质谱法检测各组胎鼠脑组织牛磺酸水平.结果 正常对照组、IUGR组与牛磺酸组胎鼠平均出生体质量分别为(6.619 ±0.413)g、(4.509±0.454)g、(5.176 ±0.436)g,组间差异有统计学意义(F=429.818,P＜0.01).正常对照组、IUGR组与牛磺酸组胎鼠脑组织牛磺酸水平分别为(2.399 ±0.134)×103 μg/g、(1.881 ±0.166)×103 μg/g和(2.170 ±0.191)×103 μg/g,组间差异有统计学意义(F=24.828,P＜0.01).结论 孕鼠补充牛磺酸可增加IUGR胎鼠脑组织内牛磺酸水平.

abstractsObjective To examine the taurine levels and determine the influence of antenatal taurine on the taurine content in the brains of fetal rats with intrauterine growth restriction (IUGR).Methods Fifteen pregnant rats were randomly divided into 3 groups:normal control group,IUGR group and the IUGR + antenatal taurine supplement group(taurine group) (n =5).IUGR models were induced by low protein diet throughout gestation period.Taurine was added to the diet of the taurine group with a dose of 300 mg/(kg · d) from 12 days after conception until natural delivery.Two fetal rats were randomly selected from each nest and were sacrificed to obtain the brains,and the taurine levels in fetal rat brains were detected by high performance liquid chromatography-mass spectrometry.Results The average weight of fetal rats in the normal control group,the IUGR group and the taurine group were (6.619 ± 0.413) g,(4.509±0.454) g,(5.176 ±0.436) g,there was a significant difference among the 3 groups(F =429.818,P ＜ 0.01).The taurine levels in fetal rat brain in the normal control group,the IUGR group,and the taurine group were (2.399 ±0.134) × 103 μg/g,(1.881 ±0.166) × 103 μg/g and(2.170 ±0.191) × 103 μg/g,there was a significant difference among the 3 groups(F =24.828,P ＜ 0.01).Conclusion Antenatal taurine supplementation can significantly increase the taurine level in fetal rat brain with IUGR.