摘要目的 探讨转化生长因子-β1 (TGF-β1)与肥胖相关性肾病(ORG)的联系,及其在疾病发生发展过程中的作用和可能的治疗方案.方法 30只SD大鼠按体质量随机分为2组,对照组(15只)以正常饲料喂养,ORG模型组(15只)以高脂高能量饲料饲养,8周末将所有大鼠处死并取其双肾,行免疫组织化学定性检测肥胖大鼠肾脏TGF-β1的表达;实时荧光定量(RT)-PCR提取并检测TGF-β1 mRNA表达,进行半定量分析,采用Western blot检测TGF-β1蛋白表达情况.结果采用SPSS 13.0软件进行统计学处理.结果 与对照组比较,ORG模型组肾组织免疫组织化学结果显示TGF-β1定性表达增多,主要表达于肾小管及肾间质部位,对照组、OR模型组平均吸光度值分别为0.040±0.013、0.171 ±0.084,差异有统计学意义(P ＜0.05);RT-PCR结果提示ORG模型组TGF-β1 mRNA表达较对照组增多(4.4比0.6),差异有统计学意义(P＜0.05);Western blot检测ORG模型组TGF-β1蛋白表达水平高于对照组(4.3比0.4),差异有统计学意义(P=O.002).结论 ORG大鼠肾脏TGF-β1表达水平升高,该因子参与了ORG的疾病发展过程,为ORG的发病机制及可能的治疗提供依据.

abstractsObjective To explore the relationship between transforming growth factor-β1 (TGF-β1) and obesity-related glomerulopathy (ORG),and to analyze the possible mechanism for ORG and the new approach to its treatment.Methods Based on their body weight,30 SD rats were randomly divided into 2 groups : the normal control group (15 rats) fed with common food and the ORG model group (15 rats) fed with fat-enriched diets.The rats were sacrificed at the end of the 8th week,and their kidneys were taken out.Immunohistochemistry was used to detect TGF-β1 protein expression.Real time (RT)-polymerase chain reaction (PCR) was used to extract and detect the expression of TGF-β1 mRNA,and Western blot was applied to examine the expression of TGF-β1 protein.The findings were analyzed by using SPSS 13.0 software.Results Compared with the control group, qualitative TGF-β1 expression in ORG model group were significantly increased detected by immunohistochemistry mainly in renal tubules and interstitium.The average absorbance value of the control group and the model ORG group was 0.040-0.013,0.171 ± 0.084, respectively.The difference was statistically significant(P ＜ 0.05).The expression of TGF-β1 mRNA detected by RT-PCR was also increased compared with that of the control group(4.4 vs 0.6).The difference was statistically significant(P ＜ 0.05).The protein expression of TGF-β 1 examined by Western blot showed that it was more than that in the control group(4.3 vs 0.4).The difference between the control group and ORG model group was statistically significant(P =0.002).Conclusions The expression of TGF-β 1 in kidneys of ORG model rats increased, which not only indicates it can participate in ORG's occurrence and development, but also provide the basis to find out the mechanism and the approach to treatment.