摘要Pigs lack functional uncoupling protein 1 (UCP1) making them susceptible to cold.Nevertheless,several pig breeds are known to be cold resistant.The molecular mechanism(s) enabling such adaptation are currently unknown.Here,we show that this resistance is not dependent on shivering,but rather depends on UCP3 and white adipose tissue (WAT) browning.In two cold-resistant breeds (Tibetan and Min),but not a cold-sensitive breed (Bama),WAT browning was induced after cold exposure.Beige adipocytes from Tibetan pigs exhibited greater oxidative capacity than those from Bama pigs.Notably,UCP3 expression was significantly increased only in cold-resistant breeds,and knockdown of UCP3 expression in Tibetan adipocytes phenocopied Bama adipocytes in culture.Moreover,the eight dominant pig breeds found across China can be classified into cold-sensitive and coldresistant breeds based on the UCP3 cDNA sequence.This study indicates that UCP3 has contributed to the evolution of cold resistance in the pig and overturns the orthodoxy that UCP1 is the only thermogenic uncoupling protein.