Expression of Nonfusion Extracellular Porcine Zona Pellucida Protein 3β in E. coli

二维码有效期 120s

摘要Objective To obtain the recombinant nonfusion extracellular porcine zona pellucida protein 3β (pZP3β ) in E. coliMethods By modificated the transition initiation region (TIR) in primers, synthetic nucleotide was gained by PCR. Such gene was cloned into pET-3c vector and trans-formed into E. coli BL21(DE3)pLysS.Results The recombinant nonfusion extracellular pZP3β was expressed in E. coli to 10% of total cellular proteins, and identified by the Western blot method.Conclusion Modification of nucleotide without changing amino acid sequences is an effective means to increase non fusion expression rate of recombinant proteins, such as pZP3β in E. coli.

作者单位 Bioengineering Institute, Jinan University, Guangzhou 510632, China ^[1] Center for Reproductive Immunology Research, Jinan University, Guangzhou 510632, China ^[2] Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China ^[3] Medical College, Jinan University, Guangzhou 510632, China ^[4]

关键词 nonfusion extracelluar porcine zona pellucida gene expression

分类号 S8

发布时间 2005-11-17

基金项目

the Science & Technology Plan

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