摘要Objective To investigate the feasibility of vitrification of blastocysts following blastomere biopsy.Methods Among patients undergoing pre-implantation genetic diagnosis(PGD),artificial shrinkage of the blastocoelic cavity and subsequent vitrification of applicable surplus blastocysts after day-3 blastomere biopsy were performed.According to patient requirements,thawed blastocysts were transferred into patients due to pregnancy failure after fresh embryo transfer,ectopic pregnancy,ovarian hyperstimulation.Results Twenty-ur PGD cycles were carried out.According to genetic diagnosis and the development of blastocysts,transfer was cancelled in 7 cycles due to absence of applicable embryos or ovarian hype rstimulation.In the remaining 17 cycles,26 blastocysts were thawed and transferred,which resulted in 13 implanted(50.0%).Clinical pregnancies were observed in 11 patients(64.71 %).Following transfer,30 applicable blastocysts in 10 cycles were cryopreserved.Six patients received transfer of thawed blastocysts.All 8 thawed embryos survived and were transferred,and singleton pregnancies occurred in 5 patients.Two women delivered healthy infants and 3 pregnancies are ongoing.Conclusion Vitrification with artificial shrinkage is effective for preserving blastocysts following blastomere biopsy.