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Effects of Cryopreservation on Human Sperm Glycocalyx

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摘要Background: To study the effects of cryopreservation on human sperm glycocalyx. Methods: The lectin binding profilings of sperm after freeze-thaw were compared by lectin microarray. Results: CryoSperm? and direct fumigation were confirmed to be the optimized cryoprotectant and method by comparing the sperm recovery rate. In 91 lectins, 33 lectins were significantly changed after sperm cryopreservation. Among them, 9 lectins greatly decreased and 24 lectins mainly increased. The binding signals of MAA, PSA,ABA, andAIAwere verified by FACS, and the results were consistent with that of lectin microarray. Conclusions: Sperm glycocalyx had significant changes after cryopreservation. The sialic acid, playing an important role in protecting sperm, was greatly lost, which exposed the inner carbohydrates. Thus, the glycocalyx damage due to the cryopreservation might be one of the reasons for low sperm fertility.

作者单位 Department of Applied Biology, State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China ^[1] Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Shanghai Ji Ai Genetics and IVF Institute, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China ^[2] Department of Allergy, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200001, China ^[3] Department of Reproductive Pharmacology, NPFPC Key Laboratory of Contraceptives and Devices, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China ^[4] Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 201100, China ^[5] Department of Andrology, Urologic Medical Center, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai 200080, China ^[6] Department of Applied Biology, State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China;Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China ^[7]

关键词 Cryopreservation Damage Glycocalyx Lectin Microarray Sperm

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Original Articles

DOI 10.4103/2096-2924.224914

发布时间 2018-04-23（万方平台首次上网日期，不代表论文的发表时间）

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