摘要目的:探讨转录因子Emx2和Emx2反义非编码长链RNA(EMX2 opposite strand/antisense RNA,Emx2OS)在小鼠生殖嵴发育过程中的表达模式以及对原始生殖细胞发育的作用.方法:分别收集孕11.5～14.5 d小鼠的生殖嵴,通过原位杂交、qRT-PCR和免疫荧光方法,观察分析Emx2和Emx2OS在生殖嵴中的表达定位及其表达水平的变化.结果:在孕11.5～14.5 d雌性和雄性小鼠胚胎生殖嵴中均检测到Emx2OS的表达,而且其表达模式与Emx2高度一致;在小鼠胚胎原始生殖细胞减数分裂的启动以及性别决定的时间点(孕12.5 d),雌性胎小鼠生殖嵴中Emx2和Emx2OS的表达量都显著上调(P＜0.001).结论:Emx2和Emx2OS在胎小鼠生殖嵴中呈同步表达模式,并可能参与调控原始生殖细胞减数分裂的启动.

abstractsObjective:To study the expression patterns of Emx2 and Emx2OS in female or male mouse gonads.Methods:Mouse gonads from 11.5 d to 14.5 d were collected.In situ hybridization and qRT-PCR were used to detected the localization and expression trend of Emx2OS.Results:Emx2OS was expressed in the female or male mouse gonads.In addition,Emx2 and Emx2OS transcripts showed remarkable concordance at each stage (from 11.5 d to 14.5 d) in both female and male gonads.At the stage of meiosis entry and the sex determination (12.5 d),the expression of Emx2 and Emx2OS was increased obviously in female gonads (P＜0.001).Conclusions:Emx2 and Emx2OS show a synchronous expression pattern in mouse gonads and may play an important role in meiosis starting regulation.