摘要目的 探讨精子线粒体膜电位(MMP)与精子常规参数及体质量指数(BMI)的相关性.方法 在本院辅助生殖科行精液分析的男性患者,禁欲3～7 d后,以手淫方式获取精液样本.通过计算机辅助精液分析仪检测精液常规,改良巴氏染色检查精子形态,JC-1染色后经流式细胞仪评估MMP.结果 与对照组(56.68％±11.13％)相比,弱精子组MMP(41.24％±9.71％)显著降低.MMP与BMI呈显著负相关(r=-0.25,P＜0.01),与精子总活力(r=0.63,P＜0.01)、前向运动力(r=0.64,P＜0.01)及正常精子形态率(r=0.37,P＜0.01)呈显著正相关,而与年龄、精液量、精子浓度和精子数量其余参数的相关性均无统计学意义.结论 精液中精子MMP是评估精子功能的重要指标,对临床综合分析男性不育症因素具有一定的参考意义.

abstractsObjective To investigate the relationship among sperm mitochondrial membrane potential (MMP),semen parameters and body mass index (BMI).Methods Semen samples were obtained by masturbation after 3-7 d of sexual abstinence from all men in semen collect room of Shanghai Ninth People's Hospital.Conventional semen analyses were performed by computer-assisted sperm analysis (CASA),and sperm morphology was analyzed by modified Papanicolaou staining.Spermatozoa were stained by JC-1 to evaluate the MMP by flow cytometry.Results Sperm MMP of asthenozoospernic group (41.24％ ± 9.71％) was significantly lower than that in the control (56.68％ ± 11.13％).MMP was negatively correlated with BMI (r=-0.25,P＜0.01),but had instead a positive correlation with sperm total motility (r=0.63,P＜0.01),progressive motility (r=0.64,P＜0.01) and normal sperm morphology (r=0.37,P＜ 0.01),respectively.And there was no statistically different correlation among MMP,age,volume of semen,sperm concentration,and sperm count.Conclusion Sperm MMP was an important index to evaluate the function of sperm,which has a certain reference value for clinical comprehensive analysis of male infertility.