摘要体外激活（ in vitro activation，IVA）技术是指IVA原始卵泡，使其生长至可接受激素刺激的阶段，结合促排卵获得成熟卵泡的技术，最终通过体外受精-胚胎移植（ in vitro fertilization and embryo transfer，IVF-ET）技术达到受孕目的。常用方法为激活PI3K/Akt信号通路和阻断Hippo信号通路。随着研究深入，新的激活方法逐渐发展。现已有多例早发性卵巢功能不全（primary ovarian insufficiency，POI）患者应用IVA技术成功妊娠的案例报道，结合生育力保存技术，卵巢低反应（poor ovarian response，POR）以及女性恶性肿瘤患者都可能受益于IVA技术。然而IVA技术尚不完善，仍在临床成功率、安全性等方面存在问题，需要在机制和临床应用继续探索。本文针对IVA技术的机制、临床应用情况以及发展方向方面进行论述，为IVA的研究和临床应用提供参考。

abstractsIn vitro activation (IVA) technology refers to the process of activating primordial follicles to grow to a stage that can be stimulated by hormones in vitro, combined with the technology of controlled ovarian hyperstimulation to obtain mature follicles, and finally achieve the purpose of conception through in vitro fertilization and embryo transfer (IVF-ET) technology. The common methods are activating the PI3K/Akt pathway and disrupting the Hippo pathway. With the deepening of research, new activation methods have gradually developed. Multiple cases of successful pregnancy with IVA technology have been reported in patients with primary ovarian insufficiency (POI). Combining with fertility preservation technology, patients with poor ovarian response (POR) and female malignant tumors may all benefit from IVA technology. However, the IVA technology is not perfect, and there are still problems in clinical success rate, safety and other aspects, and it is necessary to continue to explore the mechanism and clinical application. In this paper, the mechanism, clinical application and development direction of IVA technology were discussed, so as to provide reference for the research and clinical application of IVA.