摘要Traditionally oocytes have been exposed to sperm overnight for 16-20 h.This long period of co-incubation,however,has been shown to create problems with high levels of reactive oxygen species (ROS),which may affect embryo viability and cause hardening of the zona pellucida.Recently,several positive and negative effects of reducing the co-incubation time to 90-120 min were reported.The objective of this review was to discuss whether short exposure of oocytes to sperm in vitro might affect the clinical results and several problems arose in short gamete co-incubation.
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