换一批
换一批摘要To investigate the technique of sorting high-purity precartilaginous stem cells from rat's perichondrium, neonatal rat's perichondrium cells suspensions were incubated with monoclone antibody of anti-fibroblast growth factor receptor-3 (anti-FGFR-3), and the labeled cells were separated from the suspension in the magnetic field by immuno-beads coated with the second antibody. Purityof the sorted neural stem cells was found to be 93.0 %-99.0 %, with living cells amounting to 80 %-85 %. The magnetic cell sorting system could effectively separate precartilaginous stem cells fromperichondrium cell suspensions.
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关键词
magnetic beadsprecartilaginous stem cell (PCSC)anti-fibroblast growth factor receptor-3 (anti-FGFR-3)
分类号
R3
发布时间
2007-03-13
基金项目
国家自然科学基金
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