摘要AIM To study the effect of acupuncture on the IL2-IFN-NKC immunoregulatory system and tumorinhibition of HAC grafting hepatocarcinoma mice models, and to provide the latest theoretical evidence ofcuring diseases immunologically with acupuncture.METHODS The 28 HAC-vaccinated BALB/C mice were randomly divided into a tumor-bearing controlledgroup and tumor-bearing group to be needed. For the latter group, the selected acupoints are Dazhui (DU,14) and Zusanli (St, 36) which are localized according to the corresponding positions indicated in thecomparative anatomy of animals. The mice in the group were needled once a day for 12 days with thesupplementing and reducing methods, the frequency being 60 times/minute and the needles being retained for2 minutes. The former group was treated in the same way as the latter except needling. Twenty-four hoursafter the last needling, the mice were killed and the spleen taken out to prepare a cell suspension at requiredconcentration, The IL-2 (MTT method) and NKC (colorimetric method) were determined respectively; theIFN (CPE micr-oplate staining) was determined by using the serum separated from the mice's orbit blood; thetumor mass was taken and weighed with an analytical balance (1/1000) to calculate the tumor inhibitationrate according to the formula.RESULTS In the tumor-bearing group after having been needled. the activity of IL-2 (OD value), the titerof IFN (U/mL), the soluble rate of NKC (%) and the tumor weight were respectively 1. 3542±0. 226, 50±0.12, 33.19±4.01 and 70.88±22.8, while those corresponding items in the controlled group were 1.1049±0.028, 3.1±0.22, 21.58±2.77 and 112.45±21.1, When all the concerned items were compared with, thedifference was obvious (P<0.01), showing the IL-2, NKC and IFN of the tumor-bearing group after beingneedled were greatly increased, and the tumor weight is much decresed (inhibitation rate 43.06%). Thedifference between groups was studied by Student's t test.CONCLUSION Acupuncture can strengthen the positive immunoregulatory function of the IL2-IFN-NKCsystem of the HAC bearing mice with hypoimmunity.