摘要Malignant melanoma,characterized by invasive local growth and early formation of metastases,is the most aggressive type of skin cancer.Melanoma inhibitory activity(MIA),secreted by malignant melanoma cells,interacts with the cell adhesion receptors,integrins α4β1 and α5β1,facilitating cell detachment and promoting formation of metastases.In the present study,we demonstrate that MIA secretion is confined to the rear end of migrating cells,while in non-migrating cells MIA accumulates in the actin cortex.MIA protein takes a conventional secretory pathway including coat protein complex Ⅰ(COPⅠ)-and coat protein complex Ⅱ(COPⅡ)-dependent protein transport to the cell periphery,where its final release depends on intracellular Ca2+ions.Interestingly,the Ca2+-activated K+-channel,subfamily N,member 4(KCa3.1),known to be active at the rear end of migrating cells,was found to support MIA secretion.Secretion was diminished by the specific KCa3.1 channel inhibitor TRAM-34 and by expression of dominantnegative mutants of the channel.In summary,we have elucidated the migration-associated transport of MIA protein to the cell rear and also disclosed a new mechanism by which KCa3.1 potassium channels promote cell migration.