换一批摘要It is controversial whether pig pluripotent stem cells can be derived and stably maintained in a culture dish.In a recent paper published in Cell Research,Zhi et al.perform a comprehensive transcriptome landscape analysis of pig embryos at all developmental stages from embryonic(E)day 0 to E14 and identify a culture cocktail to stabilize pig pregastrulation epiblast stem cells(pgEpiSCs)in vitro for long-term passaging;the pgEpiSCs can undergo multiple rounds of gene editing and generate cloned animals.
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