摘要目的 探讨层流手术室术中手术器械的安全使用时段.方法 选择手术时间持续8h或以上的Ⅰ类手术10例,对术前空气,术前及术中4,6,8h的手术器械以及手术人员的手进行采样,采样标本进行细菌培养.结果 术前空气、术中手术人员的手及手术器械均无细菌生长,合格率为100％.结论 在达到100级层流条件的手术室,同一台无菌手术的术中器械可以安全使用8h.

abstractsObjective To investigate the safe time of sterile equipments used in laminar flow operating room.Methods In ten operations ( Class Ⅰ ) which last 8 or more hours,we sampled preoperative air,surgery equipments 4 h,6 h,8 h pre and intra-operatively,and surgical personnel' s hands for bacterial culture.Results There was no growth of bacteria in the sampling of preoperative air,surgical personnel's hands and surgical instruments during operation,pass rate was 100％.Conclusions The sterile equipments used in the same operation could be used safely for 8 hours in operating room where the condition of Level 100 laminar flow reached.