摘要目的 探讨中华眼镜蛇毒F组分抑制血小板聚集的作用机制.方法 用比浊法测定中华眼镜蛇毒F组分对二磷酸腺苷、花生四烯酸和血小板活化因子诱导血小板聚集作用的影响,流式细胞术观察中华眼镜蛇毒F组分对荧光标记的单克隆抗体CD41(FITC-CD41)和CD61(FITC-CD61)与血小板膜糖蛋白Ⅱb/Ⅲa(GPⅡb/Ⅲa)结合的影响.结果 中华眼镜蛇毒F组分明显抑制二磷酸腺苷、花生四烯酸和血小板活化因子诱导的血小板聚集,其作用呈现一定程度的剂量依赖关系.中华眼镜蛇毒F组分可以明显降低单克隆抗体CD41(抗GPⅡb)与血小板的结合率,而对单克隆抗体CD61(抗GPⅢa)与血小板的结合率没有影响.结论 中华眼镜蛇毒F组分可以抑制多种激动剂诱导的血小板聚集,其机制和中华眼镜蛇毒F组分与血小板膜糖蛋白Ⅱb/Ⅲa复合物的结合有关.

abstractsObjective To explore the inhibitory mechanism of fraction F of Naja naja atra venom on platelet aggregation. Methods Nephelometery was used to detect the effect of fraction F of Naja naja atra venom on platelet aggregation induced by adenosine diphosphate (ADP), arachidonic acid (AA) and platelet activating factor (PAF). The influence of fraction F of Naja naja atra venom on the binding of fluoreacenceconjugated monoclonal antibody CD41 (FITC-CD41) and CD61 (FITC-CD61) to membrane glycoprotein Ⅱb/Ⅲa (GPⅡb/Ⅲa) of platelet was measured by flow cytometry. Results Fraction F of Naja naja atra venom significantly inhibited the aggregation of platelet induced by ADP, AA and PAF in a dose-dependent manner. Fraction F of Naja naja atra venom obviously decreased the binding of FITC-CD41 to GPⅡb, and had no effect on the binding of FITC-CD61 to GPⅢa. Conclusion Fraction F of Naja naja atra venom can inhibit the platelet aggregation induced by different agonists, which is one of mechanisms underlying fraction F of Naja naja atra venom binding to GPⅡb/Ⅲa of platelet.