摘要目的 采用肾组织连续切片探索组织三维实体构建的方法和可行性.方法 C57/BL/6J小鼠,腹主动脉灌流同定,垂直于肾长轴取组织块,锇酸后固定,Epon 812包埋,获取2.5 μm半薄连续切片400张,所有切片采用甲苯胺蓝染色.数字显微图像摄取后进行配准,检验配准正确性,之后进行色彩校正并转化为灰度图像,并做导入前预处理.最后用MIMICS软件进行肾组织三维构建.结果 间隔采用拍摄的400张光学显微切片图像作为原始数据,通过主观和客观两方面验证配准结果符合三维重构要求,使用MIMICS软件获得肾组织三维实体,三维实体的体积为51 205 497.02μm3,表面积为18 650 385.47μm2.生成的三维实体文件可以用于有限元分析.其组织微细结构经形态学分析正确.结论 利用本方法生成的三维实体不仅可以用于可视化、形态学参数的测量等,还可以构建有限元分析的几何模型.

abstractsObjective To explore a feasible method for three diamensions(3D) entity reconstruction of kidney tissue based on serial light microscopic sections. Methods C57/BL/6J mice kidneys were fixed by perfusion via abdominal aorta. Tissue blocks were cut out perpendicular to the longitudinal axis of the kidney, postfixed in OsO4, and embedded in flat molds in Epon 812. Four hundred serial sections with 2.5 μm in thickness were generated using a microtome and stained with toluidine blue. Then, images from digital microscopy were captured, aligned and checked. These images underwent color correction and were transformed into grey-scale pictures and pre-processed before imported for further analysis. Finally, 3D reconstruction of kidney was generated using the MIMICS toolkit. Results Every two of the 400 images were used as raw data, aligned and checked for the quality of 3D reconstruction from objective and subjective aspects. The 3D reconstruction entity was obtained using MIMICS, with its volume being 51 205 497.02 μm3 and surface area being 18 650 385.47 μm2. Such generated 3D entity data could be done with finite element analysis (FEA). The tissue microstructure of the entity was morphologically confirmed to be correct. Conclusions The method for 3D reconstructing renal entity not only provides an approach for visualization and morphology measurement of the tissue, but can also be useful for geometric model construction of FEA.