摘要目的 研究IgA肾病患者血清肽谱的差异性,筛选IgA肾病早期诊断的生物标记.方法 选取解放军第一八一医院肾脏科2008年8月至2009年3月就诊的患者或体检的健康人为研究对象,分为试验组和对照组,试验组为33例IgA肾病患者,对照组为10例健康志愿者、12例微小病变、10例局灶硬化和10例膜性肾病患者.ClinProt系统下采用磁珠浓缩和基质辅助激光解吸电离-飞行时间-质谱技术分析IgA肾病组与其他各组研究对象血清肽谱的差异.结果 分别筛选出5个、5个、3个、3个差异性多肽作为诊断IgA肾病与健康者、微小病变、局灶硬化、膜性肾病的潜在生物标记.结论 应用蛋白质组学技术成功建立了IgA肾病患者血清肽谱的诊断模型,为蛋白质组学技术用于IgA肾病的早期诊断提供了新的思路.

abstractsObjective To study the variabilities of serum peptidomics spectra in patients with IgA nephropathy (IgAN) and to screen for biomarkers in the early diagnosis of IgAN. Methods Patients with IgAN or other kidney diseases and healthy individuals registered to the Department of Nephrology, No. 181 Hospital of Chinese People' s Liberation Army, from August 2008 to March 2009 were recruited in this study, who were divided into the study group and the control groups. The study group included 33 patients with IgAN (IgAN group), while the control groups included 10 healthy volunteers, 12 patients with minimal change nephrotic syndrome (MCNS) , 10 with focal segmental glomerulosclerosis (FSGS) and 10 with membranous nephropathy (MN). Magnetic bead enrichment and MALDI-TOF-MS were used to analyze the variabilities of serum peptidomics spectra between IgAN group and other groups based on ClinProt system.Results A total of 5, 5, 3 and 3 differential peptide peaks, respectively, were selected as potential biomarkers for diagnosis of IgAN and healthy volunteers, MCNS, FSGS and MN. Conclusion A serum peptide fingerprint model based on proteomics technology is successfully established for diagnosis of IgAN,which may provide a novel view to understand application of proteomics in early diagnosis of IgAN.