摘要目的:探讨miR-144-3p对经过放射治疗后的前列腺癌细胞增殖和侵袭的影响及作用机制。方法:收集2020年3月至2023年5月山西省肿瘤医院收治的20例前列腺患者的癌组织及其相应癌旁组织标本。实时定量PCR（RT-qPCR）法检测miR-144-3p和RUNX1的在前列腺癌组织与癌旁组织中的表达量；Pearson法分析前列腺癌组织中miR-144-3p和RUNX1表达量的相关性。采用脂质体转染的方式将miR-144-3p寡核苷酸模拟物，miR-144-3p抑制物，miR-144-3p阴性对照组（无义序列）分别转染前列腺癌细胞DU-145，并用0.4 Gy剂量进行细胞照射，采用RT-qPCR检测细胞转染效率，采用免疫印迹检测转录因子RUNX1表达，采用细胞计数试剂盒（CCK-8法）检测每个组别细胞的增殖情况，采用Transwell实验检测每个组别细胞的侵袭能力，采用荧光素酶报告基因实验验证miR-144-3p和RUNX1之间的作用关系。结果:上调miR-144-3p的表达量能够明显抑制放疗后肿瘤细胞的增殖及侵袭，同时下调RUNX1转录因子的表达水平，但沉默miR-144-3p后能够明显促进前列腺癌细胞的增殖及侵袭，同时促进RUNX1转录因子的表达，miR-144-3p和RUNX1呈负相关的关系，表明miR-144-3p在放疗过程起到重要的作用。结论:在前列腺癌中，miR-144-3p通过调控转录因子RUNX1的表达水平抑制肿瘤细胞的增殖、侵袭，从而增加细胞放射治疗效果。

abstractsObjective:To investigate the effects of miR-144-3p on the multiplication, aggression, of prostatic carcinoma cells after radiotherapy and its potential mechanisms.Methods:Cancer tissue and corresponding adjacent tissue specimens were collected from 20 prostate cancer patients admitted to Shanxi Cancer Hospital from March 2020 to May 2023. Real-time quantitative PCR (RT-qPCR) was used to measure the expression levels of miR-144-3p and RUNX1 in prostatic carcinoma tissue and adjacent tissue. Pearson correlation analysis was performed to analyze the correlation between the expression levels of miR-144-3p and RUNX1 in prostate cancer tissue. Prostate cancer cells DU-145 were transfected with miR-144-3p mimics, miR-144-3p inhibitors, and miR-144-3p negative control (non-targeting sequence) using liposome transfection method, and then irradiated with a dose of 0.4 Gy. RT-qPCR was used to detect the transfection efficiency of cells, Western blot was performed to examine the expression of transcription factor RUNX1, cell proliferation was assessed using the Cell Counting Kit-8 (CCK-8) assay, cell invasion abilities were evaluated using the Transwell assay, and the interaction between miR-144-3p and RUNX1 was validated using a luciferase reporter gene assay.Results:Up-regulating the expression of miR-144-3p can significantly inhibit the multiplication, aggression of prostatic carcinoma cells after radiation therapy, and downregulated the expression level of transcription factor RUNX1. However, silencing miR-144-3p significantly promoted cell multiplication, aggression of prostatic carcinoma cells and upregulated the expression of RUNX1. There was a negative correlation between miR-144-3p and RUNX1, indicating the important role of miR-144-3p in radiotherapy.Conclusion:The upregulation of miR-144-3p can exert inhibitory effects on the cell multiplication, aggression of prostatic carcinoma cells by suppressing the expression of the transcription factor RUNX1, thereby enhancing the efficacy of radiotherapy on these cells.