摘要目的:探讨超声引导下经皮经肝门静脉穿刺注射吲哚菁绿在腹腔镜解剖性肝切除术中的应用价值。方法:采用回顾性描述性研究方法。收集2022年10月至2024年5月福建医科大学孟超肝胆医院收治的17例肝细胞癌行腹腔镜解剖性肝切除术男性患者的临床病理资料；年龄为59（27~74）岁。患者均由超声引导下经皮经肝门静脉穿刺注射吲哚菁绿，行吲哚菁绿荧光引导腹腔镜解剖性肝切除术。观察指标：（1）手术情况。（2）术后情况。（3）随访情况。正态分布的计量资料以 x±s表示，偏态分布的计量资料以 M（范围）表示。计数资料以绝对数表示。 结果:（1）手术情况。17例患者均成功完成超声引导下经皮经肝门静脉穿刺注射吲哚菁绿，穿刺注射时间为6（3~12）min。荧光染色均成功显示目标肝段，未发生浸染。荧光染色边界距离肿瘤边缘均>1.0 cm，与术前三维重建成像基本吻合。17例患者中，11例穿刺1支门静脉分支，4例穿刺2支门静脉分支，2例穿刺3支门静脉分支。17例患者手术时间为（229±51）min，术中出血量为200（100~300）mL，术中无输血，无中转开腹手术。（2）术后情况。17例患者中，2例发生Clavien-Dindo Ⅰ~Ⅱ级并发症，均为少量胸腔积液，未经特殊处理，自行吸收好转。17例患者术后住院时间为5（5~6）d，术后病理学检查结果均为肝细胞癌，标本切缘均为阴性，肿瘤长径为4.5（1.8~12.5）cm。（3）随访情况。17例患者均获得随访，随访时间为14（2~30）个月，均未出现肿瘤复发。结论:超声引导下经皮经肝门静脉穿刺注射吲哚菁绿应用于腹腔镜解剖性肝切除术安全、可行。

abstractsObjective:To investigate the application value of indocyanine green (ICG) injection via percutaneous transhepatic portal vein under ultrasound guidance during laparoscopic anatomical liver resection.Methods:The retrospective and descriptive study was conducted. The clinicopatho-logical data of 17 male patients with hepatocellular carcinoma who underwent laparoscopic anatomical liver resection in Mengchao Hepatobiliary Hospital of Fujian Medical University from October 2022 to May 2024 were collected. The patients were aged 59(range, 27-74)years. Patients underwent ICG fluorescence-guided laparoscopic anatomical liver resection after ICG injection via percutaneous transhepatic portal vein under ultrasound guidance. Obsevation indicators: (1)surgical situations; (2) postoperative situations; (3) follow-up. Measurement data with normal distribution were represented as Mean± SD. Measurement data with skewed distribution were represented as M(range). Count data were represented as absolute numbers. Results:(1) Surgical situations. All 17 patients were successfully punctured and injected with ICG via percutaneous transhepatic portal vein under ultrasound guidance, with a puncture time of 6(range,3-12)minutes. The fluorescence staining successfully displayed at the target liver segment without infiltration, and the distance from the fluorescence staining border to the tumor edge was greater than 1.0 cm, which was consistent with preoperative three-dimensional reconstruction imaging. Among the 17 patients, 11 cases had punctures in one branch of the portal vein, 4 cases had punctures in two branches, and 2 had punctures in three branches. The operation time was (229±51)minutes, volume of intraoperative blood loss was 200(range, 100-300)mL. No blood transfusion or conversion to open surgery was required during the operation. (2) Postoperative situations. Two of 17 patients had a small pleural effusion of grade Ⅰ-Ⅱ of Clavien-Dindo classification, and were recovered without treatment. The duration of postoperative hospital stay was 5(range, 5-6)days. Postoperative pathological examina-tion showed hepatocellular carcinoma with negative surgical margin. The tumor diameter was 4.5(range, 1.8-12.5)cm. (3) Follow-up. All the 17 patients were followed up for 14(range, 2-30)months, without tumor recurrence.Conclusion:Injection of ICG via percutaneous transhepatic portal vein under ultrasound guidance during laparoscopic anatomical liver resection is safe and feasible.